The union binding sites of a leader is an extended site obtained by combining
binding site residues of each member in the 90% seq. similar family.
Individual binding sites were calculated using a 4.5 Å distance cutoff around
the ligand atoms. To better represent binding sites in their functional environment,
biological assemblies (biounits) were used, instead of asymmetric units in PDB.
We have used the ProBiS algorithm, in order to create the residue correspondences
between leader and family members. These correspondences were used to extend the
leader binding site into a union of all family members binding site.
ProBiS ref: Konc, J. & Janežič, D.; Bioinformatics 26, 11601168 (2010).